Proceedings from the 23rd March 2007 meeting in Hertfordshire, UK

“Nitric oxide (NO), generated by the enzyme nitric oxide synthase (NOS) plays a key role in a diverse range of physiological and pathophysiological conditions within the cardiovascular, immune, reproductive and nervous systems. Since NO is a small, diffusible, highly reactive free radical with a short half-life, and is present in low concentrations, real-time detection of NO is extremely difficult. This meeting examined methods used to detect and visualise NO-producing cells. Furthermore, direct and indirect techniques used to measure NO were examined, including among others, the measurement of NOS enzymatic activity, electron paramagnetic resonance (EPR) and NO-sensitive electrodes. Finally, tools that facilitate NO research, such as NOS inhibitors were examined”.  Meeting’s Chair: Dr Nicholas Warrick – University of Oxford


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Table of Contents






Quantification of Blood and Tissue NO Metabolites by Gas Phase Chemiluminescence: a Guide to NO Man’s Land

  • Introduction
  • Vascular Nitric Oxide Metabolism
  • Methods for Metabolite Measurement
  • Principle of Chemiluminescence
  • Methods of Sample Preparation
  • Controversy
  • Practicalities
  • Concluding Remarks
  • References

Radiochemical HPLC measurement of nitric oxide synthesis (NOS) activity in vascular tissue

EPR-based measurement of reactive nitrogen intermediates in human inflammatory diseases

Nitric oxide sensors and their biomedical applications

Understanding NO synthase inhibition; potency, selectivity and implications for their pharmacology

  • References

Endogenously occurring inhibitors of nitric oxide synthase

  • Abstract
  • Methylated arginines as NOS inhibitors
  • Measuring ADMA
  • Conditions in which ADMA is elevated
  • Arginine methylation
  • Regulation of ADMA
  • Could ADMA be causative in cardiovascular pathologies?

Evaluating endothelial function in humans: From bench to bedside

Nitric oxide synthase inhibition and renal injury

  • References

Quantification of enzyme independent stores of nitric oxide in human skin

  • References



NO quantification in abnormal and manipulated red blood cells

  • References

Application of NO detection using Fe(DETC) spin trap and ESR spectroscopy in pharmacological research

  • Halogenated volatile anestherics (HVA)
  • Flavonoids. Baicalein and luteolin
  • TO-85 and TO-133
  • Mildronate
  • References

Hypoxia-induced regulation of nitric oxide synthase in cardiac endothelial cells and myocytes

  • Methods
  • Results
  • Conclusions

Nitrite regulation of energy expenditure in man

Nitric oxide inhibits cellular lipid and protein synthesis without energy depletion: a process of chronic diseases due to nitrosothiols

NOx concentrations in human brain extracellular fluid and their relationship with energy metabolism: a pilot microdialysis study in acute brain injury

  • Background and aims
  • Patients and methods
  • Results